Aulan Science and Technology Co.
Separation, purification, and renaturation in one step
Contact us:
N. America, frankli@aulanst.com
Asia, xdgeng@aulanst.com
1. Products:
Caky chromatographic columns
Fig 1. Caky chromatographic columns, US patented products.
Specifications
|
|---|
Type |
Model |
Dimension
(height',
inner diameter
mmI. D.) |
Functional group |
Hydrophobicity |
pH |
Examples |
| Hydrophobic |
XD-FM |
5' (5-10),
10' (10-500),
20' (10-500),
30' (10-500),
40' (10-500),
50' (10-500). |
4 hydrogen-furan |
weak |
2-8 |
cytochromoid,
myoglobin,
rinonuclease,
lysozyme,
alpha-amylase,
insulin, rhINF-gamma |
|
XD-Phe |
~ |
benzyl |
medium |
2-8 |
~ |
|
XD-PEG |
~ |
hydroxyl |
medium |
2-8 |
~ |
|
XD-ETH |
~ |
modified ether |
strong |
2-8 |
~ |
| Ionic polymer |
XD-CM |
~ |
COO- |
|
6-12 |
~ |
|
XD-SP |
~ |
-N(C2H5)2 |
|
4-12 |
~ |
|
XD-DEAE |
~ |
-N(CH3)2 |
|
2-10 |
~ |
|
XD-QAE |
~ |
-(SO3)2 |
|
2-12 |
~ |
| Silicone |
XD-S-CM |
~ |
COO- |
|
6-8 |
~ |
|
XD-S-SP |
~ |
-N(C2H5)2 |
|
4-8 |
~ |
|
XD-S-QAE |
~ |
-(SO3)2 |
|
2-8 |
~ |
|
2. Functions:
The caky column performs separation, renaturation and purification of biopolymers efficiently in one step with excellent results.
3. Applications
Caky chromatographic columns (or cakes) can be used successfully at a pressure of more than 20 Mpa. Such cakes do not deform during use, which helps to insure the surface evenness of the chromatographic packing at the end of a sample injection which, in turn, enhances the resolution. Using the cakes of this product, separation is fast even with a low system pressure (e.g., lower than 5.0 Mpa generally). From the standpoint of good resolution under high flow rate conditions, the performance of chromatography cakes in accordance with this product is comparable to that of perfusion chromatography and, therefore, has superior industrial applicability. The chromatographic cakes of this product also allow for sample injections having a relatively high viscosity and a little precipitation. When the chromatographic separation is completed with the cakes of this product, no fixed inlet and outlet are needed. The cakes of this product show only a small irreversible absorption on objective products, which enhances the recovery of such objective products. With the chromatographic cake of this product, the separation, renaturation, and purification processes can be performed in one step, which makes it at least three times simpler than the normal renaturation and purification technology. With the chromatographic cakes of this product, the relevant production period can also be shortened by at least several times compared with comparable conventional processes and chromatographic devices. The investment for equipment is also reduced significantly using the chromatographic cakes of this product. At the same time, denaturants can also be recovered when using chromatographic cakes in accordance with this product. Not only can such recovered denaturants be reused, but also the environmental pollution caused by disposing of such denaturants can be reduced. A chromatographic cake in accordance with this product can play a roll of "killing four birds with one stone", (that is, quick and complete elimination of denaturants, easy recycling of denaturants, protein renaturation, and separation of impure proteins). Using only the same packing volume as a conventional chromatographic column, in comparison with normal columns, more packing material can be packed into the inner cavity of a chromatographic cake packed in accordance with this product. Relatively greater mass loading and volume loading is therefore possible with the chromatographic cakes of this product.
4. References
[1] X. D. Geng, and C. Z. Wang, Protein folding liquid chromatography and its recent developments, J. Chromatography B (2006),doi:10.1016/j.jchromb.2006.10.068
[2] X. D. Geng, Q. Bai, Y. Zhang, X. Li, and D. Wu, Refolding and purification of interferon-gamma in industry by hydrophobic interaction chromatography, J. Biotechnology,113(2004) 137-149, doi:10.1016/j.jbiotec.2004.06.006
Contact us, Tel: 858-740-0349, e-mail: frankli@starnetproduction. Update: Jul. 23, 2008
